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  • FAQ: Is SacI affected by methylation?

    SacI is sensitive to cytosine methylation at GAGmCTC but not GAGCTmC and insensitive to adenine methylation at GmAGCTC/. AluI methylase (AGmCT) can be used to block SacI. Methylation from common E. coli strains and mammalian sources wil not block SacI.

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