• My NEB
  • Print
  • PDF
  • FAQ: Is SacI affected by methylation?

    SacI is sensitive to cytosine methylation at GAGmCTC but not GAGCTmC and insensitive to adenine methylation at GmAGCTC/. AluI methylase (AGmCT) can be used to block SacI. Methylation from common E. coli strains and mammalian sources wil not block SacI.

    Links to this resource

    Products:
    SacI