FAQ: Is it necessary to treat my glycoprotein concomitantly with Neuraminidase and O-Glycosidase?

Yes. Neuraminic Acid residues must be removed in order to allow O-Glycosidase to cleave the O-linked disaccharides. A general Neuraminidase (NEB #P0720) works well. Additionally, an O-Glycosidase and Neuraminidase Bundle (NEB# E0540S) is available.