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  • FAQ: Is it necessary to gel-extract restriction fragments or PCR products?

    Gel extraction of restriction fragments is generally not necessary. A column cleanup kit (we recommend the Monarch DNA Gel Extraction Kit, NEB #T1020) or a standard phenol-chloroform extraction, followed by ethanol precipitation is sufficient. We have also used unpurified PCR products directly in assembly reactions as long as the PCR product is > 90% pure.  If you wish to purify your PCR products, we recommend the Monarch PCR & DNA Cleanup Kit, NEB #T1030.