FAQ: I noticed that the molecular weights I am observing are slightly different than those reported. Can you explain?

10–20%
Tris-glycine
4–12%
Bis-tris (MOPS)
3–8%
Tris-acetate
230 200 220
150 130 135
100 95 100
80 70* 78
60 55 60
50 45 50
40 35 38
30 27 28
25 24 NA
20 20 NA
15 13 NA
10 7 NA

Note: Apparent molecular weight values for prestained protein markers can be different when run on different gel types. Due to poor resolution, NEB #P7711 is not recommended for use on Tris-tricine or Bis-tris (with MES buffer) gels. *A second band can be observed at 165.