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  • FAQ: I noticed that the molecular weights I am observing are slightly different than those reported. Can you explain?

    10–20%
    Tris-glycine
    4–12%
    Bis-tris (MOPS)
    3–8%
    Tris-acetate
    230 200 220
    150 130 135
    100 95 100
    80 70* 78
    60 55 60
    50 45 50
    40 35 38
    30 27 28
    25 24 NA
    20 20 NA
    15 13 NA
    10 7 NA

    Note: Apparent molecular weight values for prestained protein markers can be different when run on different gel types. Due to poor resolution, NEB #P7711 is not recommended for use on Tris-tricine or Bis-tris (with MES buffer) gels. *A second band can be observed at 165.