FAQ: I notice that the molecular weights I am observing are slightly different than those reported. Can you explain?

10–20%
Tris-glycine
4–12%
Bis-tris (MOPS)
3–8%
Tris-acetate
230 200 220
150 130 135
100 95 100
80 70 78
60 55 60
50 45 50
40 35 38
30 27 28
25 24 NA
20 20 NA
15 13 NA
10 7 NA

Note:
Apparent molecular weight values for prestained protein markers can be different when run on different gel types. Due to poor resolution, NEB #P7710 is not recommended for use on Tris-tricine or Bis-tris (with MES buffer) gels.