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  • FAQ: I notice that the molecular weights I am observing are slightly different than those reported. Can you explain?

    10–20%
    Tris-glycine
    4–12%
    Bis-tris (MOPS)
    3–8%
    Tris-acetate
    230 200 220
    150 130 135
    100 95 100
    80 70 78
    60 55 60
    50 45 50
    40 35 38
    30 27 28
    25 24 NA
    20 20 NA
    15 13 NA
    10 7 NA

    Note:
    Apparent molecular weight values for prestained protein markers can be different when run on different gel types. Due to poor resolution, NEB #P7710 is not recommended for use on Tris-tricine or Bis-tris (with MES buffer) gels.