• My NEB
  • Print
  • PDF
  • FAQ: How should the NEBuffer be used?

    The buffer must be completely thawed before use. Dilute the 10X stock with dH2O to a final concentration of 1X. Add the water first, buffer next, the substrate and finally the enzyme. For example, a 50 µl reaction should contain 5 µl of 10X buffer with the rest of the volume coming from the substrate, enzyme and dH2O.