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  • FAQ: How should I express my protein of interest in SHuffle?

    For initial conditions we recommend using rich media at 30°C. Otherwise, overnight at 16°C is possible.
    At 30°C or 16°C, inoculate 1% overnight culture and grow cells at 30°C for 3 hours until OD600 ~ 0.8 and then induce expression of protein for at least 5 hours at 30°C or overnight at 16°C.
    If using 37°C, inoculate 1% overnight culture and grow cells for 2 hours at 37°C until OD600~0.8 and then induce expression of protein for at least 6 hours at 37°C.