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  • FAQ: How does the level of star activity of SphI-HF compare to SphI?

    The Fidelity Index which measures the minimum units to produce star activity was determined using the following conditions; 1X NEBuffer, 5% glycerol, lambda DNA (1µg/50 µl), incubated for 1 hour at 37˚C . The minimum number of units of SphI-HF required to produce star activity in the recommended NEBuffer 4 is (≥2,000 units). The minimum number of units of SphI required to produce star activity in the recommended NEBuffer 2 is (32 units).The minimum number of units of SphI required to produce star activity in NEBuffer 4 is (16 units).