• My NEB
  • Print
  • PDF
  • FAQ: How does the level of star activity of SalI-HF compare to SalI?

    The Fidelity Index which measures the minimum units to produce star activity was determined using the following conditions; 1X NEBuffer, 5% glycerol, lambda DNA HindIII digest (1µg/50 µl), incubated for 1 hour at 37˚C . The minimum number of units of SalI-HF required to produce star activity in the recommended NEBuffer 4 is (≥2,000 units). The minimum number of units of SalI required to produce star activity in the recommended NEBuffer 3 is (4 units).The minimum number of units of SalI required to produce star activity in NEBuffer 4 is (0.03 units).