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  • FAQ: How can I improve blunt-end ligation efficiency of PCR products?

    Vector should be treated with alkaline phosphatase and the insert should have a 5´ phosphate. Other possibilities to improve ligation include: lowering the ATP level in the ligation buffer, increasing the insert:vector ratio or trying our Quick Ligation® Kit (NEB #M2200).

    Quick Ligation® is a registered trademark of New England Biolabs, Inc.