FAQ: How can I improve blunt-end ligation efficiency of PCR products?

Vector should be treated with alkaline phosphatase and the insert should have a 5´ phosphate. Other possibilities to improve ligation include: lowering the ATP level in the ligation buffer, increasing the insert:vector ratio or trying our Quick Ligation® Kit (NEB #M2200).

Quick Ligation® is a registered trademark of New England Biolabs, Inc.