FAQ: Does plasmid size affect colony size (C2984)?

Yes. We transformed pUC19 (2.7kb) and a 10.5kb plasmid into NEB Turbo competent E.coli (NEB #C2984H) side by side following the provided 5 Minute Transformation Protocol. After overnight growth, 92 colonies existed on a plate for pUC19 transformation with colonies 2.06±0.11mm in diameter. 13 colonies were counted on a plate for a 10.5kb plasmid transformation with colonies 1.31±0.41mm in diameter. Notice that the colonies carrying the larger plasmid were smaller in size compared to pUC19.