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  • FAQ: Can the change in buffer preference of the HF enzyme be advantageous?

    All HF restriction enzymes are supplied with CutSmart® Buffer. The HF enzymes typically have a different optimal buffer than the wild type enzyme; this can be advantageous when designing double digest experiments. CutSmart Buffer was chosen as the recommended buffer for HF enzymes since it shows the highest level of enzyme compatibility in the NEB buffer system.