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  • FAQ: Can T4 DNA Polymerase be used in other NEBuffers?

    For fill-in reactions only: T4 DNA Polymerase can be used in NEBuffers 1.1, 2.1, 3.1 and CutSmart™ Buffer as well as NEBuffers 1-4 and T4 DNA Ligase Buffer.

    For blunting reactions requiring removal of overhangs: T4 DNA Polymerase can be used in NEBuffers 1.1, 2.1, and CutSmart Buffer as well as NEBuffers 1,2, and 4 and T4 DNA Lilgase Buffer. NEBuffers 3.1 and 3 are not recommended when overhang removal is required. Optimal activity is observed in NEBuffer 2.

    BSA supplementation is recommended when using a buffer that does not already contain BSA. Supplementation of dNTPs is required for all blunting reactions.