• My NEB
  • Print
  • PDF
  • FAQ: Can ssDNA oligonucleotides be combined and assembled with dsDNA fragments?

    Yes. However, the optimal concentration of each oligonucleotide should be determined. As a starting point, we recommend using 45 nM of each oligonucleotide that is less than or equal to twelve 60-base oligonucleotides containing 30-base overlaps.