FAQ: Can LB medium be used instead of SOC in the outgrowth step (C2988)?

NEB SOC outgrowth medium is made from animal-free peptone and delivers higher transformation efficiency than LB medium. All NEB competent cells (except Subcloning Efficiency NEB 5-alpha Competent E.coli [NEB#C2988J, NEB 10-beta competent E. coli [NEB#C3019][NEB#C3020K] and NEB Stable Competent E. coli [NEB#C3040]) contain SOC outgrowth medium in the kits. SOC outgrowth medium can also be purchased separately (NEB #B9020S)).