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  • FAQ: Can Klenow Fragment (3'→5' exo-) be used in labeling reactions and partial fill in reactions?

    Yes, it is the enzyme of choice for these applications. The lack of 3'→5' exonuclease activity prevents the labeled nucleotides from being removed after they are incorporated by the polymerase. The enzyme is used in the NEBlot Kit (NEB# N1500) for making high specific activity probes using random primers.