FAQ: Can I use the enzyme to de-phosphorylate proteins?

Antarctic Phosphatase can be used to remove phosphates from phosphoserine/threonine and phosphotyrosine residues in proteins. Typically 5-25 units of AnP will remove phosphate groups from 100-500 pmol of protein (5-25 ug of 50 kDa protein) in a 30 minute reaction under the recommended reaction conditions. Note that optimal incubation time and enzyme concentration must be determined empirically for a particular substrate. NEB recommends using Lambda Protein Phosphatase (NEB catalog #P0753 ), a dual protein phosphatase with a very broad specificity, to dephosphorylate proteins.