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  • FAQ: Can I use β-N-Acetylglucosaminidase in a double digest with other exoglycosidases and/or endoglycosidases?

    Yes. We recommend double digesting β-N-Acetylglucosaminidase with other exoglycosidases using 1X G6 Reaction Buffer (50mM NaAcetate, pH 5.5) (NEB #B0719S). We recommend using 1X G7 Reaction Buffer (50 mM sodium phosphate, pH 7.5) for digestion of β-N-Acetylglucosaminidase with PNGase F and/or O-Glycosidase. First digest your glycoprotein with PNGase F or O-Glycosidase, heat-kill the enzyme at 65°C for 10 min, and then treat with β-N-Acetylglucosaminidase in the same 1X G7 buffer.