FAQ: Can I use β1-4 Galactosidase in a double digest with other exoglycosidases and/or endoglycosidases?

Yes. We recommend double digesting β1-4 Galactosidase using 1X GlycoBuffer 1. We recommend using 1X GlycoBuffer 2 for digestion of β1-4 Galactosidase with PNGase F and/or O-Glycosidase. First digest your glycoprotein with PNGase F or O-Glycosidase, heat-kill the enzyme at 65°C for 10 min, and then treat with β1-4 Galactosidase in the same 1X GlycoBuffer 2.