FAQ: Can I conduct blue/white screening using alpha complementation of lacZ in SHuffle strains?

No, SHuffle strains do not have the appropriate M15 deletion in the lacZ gene for alpha-complementation. SHuffle (E. coli K12, C3026) and SHuffle express (E. coli B, C3028) cells are lacZ+, while SHuffle T7 express (E. coli B, C3029 and C3030) cells are lacZ-.