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  • FAQ: Can Taq DNA Polymerase be used for nick translation?

    Yes. The 5' flap endonuclease activity of Taq DNA Polymerase facilitates nick translation.  DNA Polymerase I (E. coli) (NEB# M0209) is another good choice for nick translation enabled by the inherent 5'→3' exonuclease activity of the enzyme.