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  • FAQ: Can DNA Polymerase I (E. coli) be used to blunt DNA?

    Yes, 5' overhangs will be filled in and 3' overhangs will be removed. However, DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210) would be the enzyme of choice for this application because it lacks the 5'→3' exonuclease activity.