FAQ: Can DNA be used as a template for M-MuLV Reverse Transcriptase?

Yes, but the reaction is less efficient (1).

(1) Gerard G. F., and D' Alessio J. M., Chapter 6 (73-93) From: Methods in Molecular Biology, Vol.16: Enzymes of Molecular Biology Edited by: M. M. Burell 1993 Humana Press Inc. Totowa, NJ