FAQ: Are Protease Inhibitors acceptable for use with the Protein Deglycosylation Mix reaction?

When a protein is denatured it is more susceptible to cleavage by proteases. For this reason a protease cocktail containing the following can be used with the Protein Deglycosylation Mix protocol:
Aprotinin (10 μg/ml final concentration)
Benzamidine (1mM final concentration)
Pepstatin (10 μg/ml final concentration)
Leupeptin (1μM final concentration)
EGTA (1 mM final concentration)
EDTA (1 mM final concentration)
PMSF (1 mM final concentration)
Make a 1000X concentrated stock of each inhibitor in water; except Pepstatin which should be dissolved in methanol.
Note: PMSF has the ability to modify basic residues on glycoprotein substrates.