HomeFAQsWill the HF enzyme produce elevated star activity when used in a buffer other than the one recommended?
FAQ: Will the HF enzyme produce elevated star activity when used in a buffer other than the one recommended?
Star activity was extensively tested in all NEBuffers that showed at least 50% enzyme activity. Star activity is significantly reduced compared to the wild type enzyme activity in all NEBuffers. However, we recommend using the suggested reaction buffer when possible because there are cases where star activity can still be problematic under extreme conditions using non-recommended buffers.