FAQ: Why isn't SmaI cutting?
2. SmaI is blocked by CpG methylation.
3. The reaction temperature for SmaI is 25°C. Incubation at 37°C gives 50% activity. SmaI has a half-life of 15 minutes at 37°C.
2. SmaI is blocked by CpG methylation.
3. The reaction temperature for SmaI is 25°C. Incubation at 37°C gives 50% activity. SmaI has a half-life of 15 minutes at 37°C.
