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New England Biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Despite a "Stay At Home" advisory being put in place in Massachusetts, USA, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. We are continuing to work with our suppliers and distribution partners to ensure uninterrupted access to our products and technical support.

Home FAQs Why isn't SmaI cutting?

FAQ: Why isn't SmaI cutting?

1. SmaI needs K⁺ to work. Use in NEBuffer 4, or supplement with KCl.
2. SmaI is blocked by CpG methylation.
3. The reaction temperature for SmaI is 25°C. Incubation at 37°C gives 50% activity. SmaI has a half-life of 15 minutes at 37°C.

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FAQ: Why isn't SmaI cutting?