HomeFAQsWhy is the product a smear when visualized on an agarose gel?
FAQ: Why is the product a smear when visualized on an agarose gel?
Taq DNA Polymerase has a half-life of 45 minutes at 94°C, therefore conditions for making long products (>5kb) can degrade the polymerase. In this case, increasing the amount of Taq DNA Polymerase in the reaction can help. Vent DNA Polymerase (NEB# M0254) and Deep Vent DNA Polymerase (NEB# M0258) are more thermostable polymerases. Q5® High-Fidelity DNA Polymerase (NEB# M0491), Q5 Hot Start High-Fidelity DNA Polymerase (NEB# M0493), Phusion® High-Fidelity DNA Polymerase (NEB# M0530), and Phusion Hot Start Flex DNA Polymerase (NEB# M0535) amplify targets up to 20 kb with extremely high fidelity. LongAmp®Taq DNA Polymerase (NEB# M0323), Crimson LongAmp Taq DNA Polymerase (NEB# M0326), and LongAmp Hot Start Taq DNA Polymerase (NEB# M0534) can amplify targets up to 30 kb in length.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.