Thanksgiving Shipping Schedule: Orders placed on Tuesday, November 22, 2022, will ship for delivery Wednesday, November 23, 2022. We will be closed on Thursday November 24, 2022, and Friday, November 25, 2022.
Orders placed on Wednesday November 23rd, will be shipped on Monday, November 28th.
HomeFAQsWhy is BciVI- cut DNA difficult to ligate?
FAQ: Why is BciVI- cut DNA difficult to ligate?
Single base-pair overhangs are poor substrates for T4 DNA ligase: they anneal very poorly, and are as difficult to ligate as are blunt ended DNA fragments. Also, for AlwI the overhang is not constrained to be any particular nucleotide; all four bases are roughly equally represented. Consequently, any given overhang is compatible with only 25% of the other overhangs present. This lowers ligation efficieny relative to overhangs generated by restriction enzymes which leave only mutually compatible overhangs. Ligation efficiency can be increased by using our Blunt/TA Ligase Master Mix.