FAQ: Which alkaline phosphatase, rSAP, CIP, or Antarctic Phosphatase, works best?

Quick CIP, in the Quick Dephosphorylation Kit (NEB #M0508) is a superior choice because it combines the advantages of both CIP (NEB #M0290) and Antarctic Phosphatase (NEB #M0289).Quick CIP has a very high specific activity comparable to CIP, but CIP cannot be completely heat inactivated. Quick CIP is heat inactivated in minutes, as is Antarctic Phosphatase, but Quick CIP is preferred over Antarctic Phosphatase because it does not require added zinc or any other co-factors. As a result, Quick CIP can be added directly to restriction enzyme digests. Additionally, after heat inactivation of Quick CIP, it is not necessary to purify vector DNA prior to the ligation reaction.