neb.com will be undergoing maintenance beginning Friday, December 9th, at 5pm ET and will last through Saturday, December 10th, at 3pm ET. Online ordering will not be available during this time. We apologize in advance for any inconvenience this may cause.
HomeFAQsWhen should I choose the HF version of the enzyme?
FAQ: When should I choose the HF version of the enzyme?
The HF version of the enzyme has the same cleavage specificity as the wild type enzyme, and should be chosen if star activity is a concern or if the recommended buffer is more convenient for double digestion or other multi-step protocol. There is no disadvantage to using the HF version.