FAQ: What is the optimal heat shock time for this strain (NEB #C3022H and NEB #C3022I)?

Heat shock at 42ºC for 10 seconds results in the highest transformation efficiency for T7 Express Crystal competent E.coli (NEB #C3022H and NEB #C3022I). Expect approximately 50% loss in transformation efficiency when heat shocking for 80 seconds (see Figure on the main product page).