FAQ: What is the optimal heat shock time for this strain (NEB #C2988J)?

Heat shock at 42ºC for 30-40 seconds results in the highest transformation efficiency for NEB 5-alpha competent E.coli (Subcloning Efficiency) (NEB #C2988J). Expect approximately 40% loss in transformation efficiency when heat shocking for 80 seconds (see Figure on the main product page).
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    Tips for Successful Transformations with NEB Competent Cells

    Follow these tips to get superior results.