HomeFAQsWhat are the functions of the buffer pack components?
FAQ: What are the functions of the buffer pack components?
DTT is present to help reduce any disulphide S-S bonds that help form secondary/tertiary structure and/or dimer formation. The SDS detergent binds to the proteins positive charges so that proteins will separate based on size and not by charge. The SDS also denatures the proteins and subunits to also help separate them based on size, not on shape.