Heparinases, also called Heparin Lyase enzymes, are enzymes that cleave the glycosidic linkage between hexosamines and uronic acids and are known to cleave heparin and HS chains selectively, via an elimination mechanism. Heparinase enzymes create a double bond on the non-reducing end of the uronic acid that absorbs at 232nm and can be used for the detection of oligosaccharide and disaccharide products.
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