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HomeFAQsIs there a compatible buffer for digesting with Trypsin (P8101S) and Endoproteinase GluC (P8100S) together?
FAQ: Is there a compatible buffer for digesting with Trypsin (P8101S) and Endoproteinase GluC (P8100S) together?
We would suggest a 50:50 mix of each enzyme's buffer. GluC works at maximal activity with a stimulating glutamic acid dipeptide in the reaction. Calcium chloride reduces autolysis of tryspin. Both buffers have a pH 8, Tris-HCI backbone. We have done our GluC/Trypsin digests this way with incubation overnight at 37°C. Neither enzyme is inhibited by the buffer mixing but they will, of course digest each other to some extent.