HomeFAQsHow much cAMP-dependent Protein Kinase (NEB# P6000) should be used?
FAQ: How much cAMP-dependent Protein Kinase (NEB# P6000) should be used?
The amount of enzyme required varies when different substrates are used. Start with 1-2 µl for 1µg of protein for one hour in a 10-25 µl reaction. If there is still unphosphorylated material, let the reaction go overnight.