HomeFAQsHow long should I incubate cells on ice after DNA has been added (NEB #C3010H and NEB #C3010I)?
FAQ: How long should I incubate cells on ice after DNA has been added (NEB #C3010H and NEB #C3010I)?
Incubating DNA with T7 Express lysY competent cells on ice for 30 minutes is recommended. Expect approximately 20% loss in transformation efficiency when incubating for 10 minutes (see Figure on the main product page).
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