FAQ: How long should I incubate cells on ice after DNA has been added (NEB #C2992H and NEB #C2992I)?

Incubating DNA with NEB 5-alpha F´Iq competent cells on ice for 30 minutes is recommended. When tested with pUC19, an incubation time of 10 minutes did not significantly lower the transformation efficiency (see Figure on the main product page).