HomeFAQsHow is the damaged DNA that you test the PreCR Repair Mix against created? Can I buy this damaged DNA separately?
FAQ: How is the damaged DNA that you test the PreCR Repair Mix against created? Can I buy this damaged DNA separately?
We have tested the PreCR Repair Mix on DNA that has abasic sites, oxidized bases, UV damage, and dU in addition to heat damaged DNA. The abasic sites are generated by incubation at pH 5 as described in Ide, H., et al (Biochemistry (1993) 32(32):8276-83). DNA oxidation is accelerated by methylene blue (Boiteux, S., et al, (1992) 31(1):106-10). UV damage by exposure to a UV light. Plasmid DNA with dU is obtained from an dut-/ung- strain of E. coli K12 CJ236 (NEB#E4141). UV damaged DNA is available from NEB only as part of the PreCR Repair Mix kit.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.