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HomeFAQsHow can I reduce the number of vector-only background colonies?
FAQ: How can I reduce the number of vector-only background colonies?
To significantly reduce the background of unwanted vector-only colonies, the vector should be a PCR product rather than a restriction fragment. If background continues to be a problem, the PCR-amplified vector can be treated with DpnI to remove the template carry-over, if applicable, extracted from an agarose gel following electrophoresis.