FAQ: Can the BioLux GLuc assay systems (NEB #E3300 & NEB #E3308) be used to assay GLuc activity in GLuc-containing blood samples and GLuc samples containing high serum?

Yes. However to analyze these data it is important to include the proper controls (GLuc assay solution alone; GLuc-containing blood sample or GLuc sample containing high serum alone, i.e., without adding the GLuc assay solution) in the assay in order to establish the background signal. The presence of serum tends to increase the background signal of the GLuc activity measurements.