FAQ: Can T7 DNA Polymerase be used in other NEBuffers, including rCutSmart?

Optimal activity is seen in T7 DNA Polymerase Reaction Buffer. It also retains at least 50% activity in standard NEB restriction buffers. It can also be used in rCutsmart Buffer. To see its % functional activity in rCutsmart, and that of other DNA Modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in rCutSmart® Buffer chart.