We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. To learn more and manage cookies, please refer to our Cookie Statement.
HomeFAQsCan T4 DNA Ligase be used in other NEBuffers, including CutSmart?
FAQ: Can T4 DNA Ligase be used in other NEBuffers, including CutSmart?
Ligation can also be performed in any of the standard restriction endonuclease NEBuffers, including CutSmart® Buffer, or in T4 Polynucleotide Kinase Buffer if supplemented with 1 mM ATP. Please be sure to use riboATP (NEB #P0756) as deoxyriboATP will not work. When using NEBuffer 3 or 3.1, high levels of salt in the DNA preparation may decrease ligation efficiency. To see its % functional activity in Cutsmart, and that of other DNA Modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in CutSmart® Buffer chart.
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
