HomeFAQsCan T4 DNA Ligase be used in other NEBuffers, including rCutSmart?
FAQ: Can T4 DNA Ligase be used in other NEBuffers, including rCutSmart?
Ligation can also be performed in any of the standard restriction endonuclease NEBuffers, including rCutSmart® Buffer, or in T4 Polynucleotide Kinase Buffer if supplemented with 1 mM ATP. Please be sure to use riboATP (NEB #P0756) as deoxyriboATP will not work. When using NEBuffer 3 or r3.1, high levels of salt in the DNA preparation may decrease ligation efficiency. To see its % functional activity in rCutsmart, and that of other DNA Modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in rCutSmart® Buffer chart.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
