FAQ: Can I use BioLux GLuc Assay Kit (NEB #E3300) to assay Renilla Luciferase activity in the cell lysate?

Since both GLuc and Renilla oxidize the same substrate, the BioLux GLuc Assay Kit can be used to assay Renilla luciferase activity. However, the lysis buffer that is used to generate the cell lysate samples has to be compatible with the BioLux GLuc Assay Kit. In order to use the BioLux GLuc Assay Kit to assay Renilla luciferase activity, we recommend that the cell lysates be prepared by using Luciferase Cell Lysis Buffer (NEB #B3321S), since this lysis buffer is designed to be compatible with Cypridina, Gaussia, Renilla, Firefly luciferase and ß-galactosidase activity assays.