HomeFAQsCan I use β-N-Acetylglucosaminidase in a double digest with other exoglycosidases and/or endoglycosidases?
FAQ: Can I use β-N-Acetylglucosaminidase in a double digest with other exoglycosidases and/or endoglycosidases?
Yes. We recommend double digesting β-N-Acetylglucosaminidase using 1X GlycoBuffer 1. We recommend using 1X GlycoBuffer 2 for digestion of β-N-Acetylglucosaminidase with PNGase F and/or O-Glycosidase. First digest your glycoprotein with PNGase F or O-Glycosidase, heat-kill the enzyme at 65°C for 10 min, and then treat with β-N-Acetylglucosaminidase in the same 1X GlycoBuffer 2.