FAQ: Can I conduct M13 phage display experiments in SHuffle strains?

No. SHuffle Express cells (C3028, C3029, C3030) do not harbor the F' episome and therefore can not be infected by M13. On the other hand, even though SHuffle T7 cells (C3026) carry the F', they are not susceptible to M13 infection. This may be due to the oxidative cytoplasm interfering with phage assembly