HomeFAQsCan I achieve digestion of a plasmid with a single SfiI site?
FAQ: Can I achieve digestion of a plasmid with a single SfiI site?
Trans reactions are aided by increasing DNA concentration (so you may achieve digestion by increasing the DNA concentration, but it may not be efficient) but, perversely, SfiI reaction can be hindered by increasing enzyme concentration. In reactions with enzyme in excess of DNA sites, each site becomes loaded with a SfiI tetramer, thus preventing the protein from binding the two sites necessary for full activity.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.