HomeFAQsCan DNA Polymerase I, Large (Klenow) Fragment be used to fill in 3' overhangs?
FAQ: Can DNA Polymerase I, Large (Klenow) Fragment be used to fill in 3' overhangs?
No, DNA polymerases cannot fill in 3' overhangs. To create blunt ends 3' overhangs must be removed. DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210), T4 DNA Polymerase (NEB# M0203) or Mung Bean Nuclease (NEB# M0250) are the best choices to remove 3' overhangs.
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