version 1.0
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NEBridge Ligase Fidelity Viewer
®
Overhang length
4-base
3-base
Ligation conditions
T4 DNA Ligase, 25°C, 1h incubation (4-base substrate)
T4 DNA Ligase, 25°C, 18h incubation (4-base substrate)
T4 DNA Ligase, 37°C, 1h incubation (4-base substrate)
T4 DNA Ligase, 37°C, 18h incubation (4-base substrate)
T7 DNA Ligase, 25°C, 18h incubation (4-base substrate)
T7 DNA Ligase, 37°C, 18h incubation (4-base substrate)
BsaI-HFv2 37-16 cycling
BsaI-HFv2 37 static
BsmBI-v2 42-16 cycling
Esp3I, 1x T4 DNA Ligase buffer, 37-16 cycling
BsaI-HFv2, 1x NEBridge Ligase MM, 37-16 cycling
PaqCI, 1x T4 DNA Ligase buffer, 37-16 cycling
PaqCI, 1x NEBridge Ligase MM, 37-16 cycling
BsmBI-v2, 1x NEBridge Ligase MM, 42-16 cycling
BbsI-HF, 1x NEBridge Ligase MM, 37-16 cycling
Overhangs (5′→3′)
Show normalized ligation counts
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