HomeFAQsDo any of the USER enzymes leave ligatable ends when cleaving at a Uracil?
FAQ: Do any of the USER enzymes leave ligatable ends when cleaving at a Uracil?
No, neither USER Enzyme (NEB #M5505), Thermolabile USER II Enzyme (NEB #M5508) nor Thermostable USER III Enzyme (NEB #M5509) will leave ligatable ends when cleaving at a uracil. However, Thermostable Endonuclease Q (NEB #M0701) will nick 5´ to a uracil (leaving the nucleobase intact), but leave ligatable 3´ OH and 5´ P ends after cleavage.
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